info@cytospector.com
Cytospector
  • Home
  • Expertise
  • High-content assays
    • Standardized phenotypic assay
    • Alzheimer´s disease assay
    • Schizophrenia assay
  • Assay development
  • About us
  • Contact
  • Home
  • Expertise
  • High-content assays
    • Standardized phenotypic assay
    • Alzheimer´s disease assay
    • Schizophrenia assay
  • Assay development
  • About us
  • Contact

Assay Development

On demand, Cytospector develops neuron-based high-content assays for compound screens and screens for therapeutic targets in the field of neurological and psychiatric disease. Combination of advanced neuronal cell models, standardized analysis of a large number of relevant neuronal and synaptic phenotypes, and our long-standing expertise with high-content screening ensures optimal assay development.

Contact us with any questions or requests for assay development.

Contact us

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Cytospector’s foundations for assay development

Primary rodent neurons

  • High-throughput, fully automated cell culture
  • Adherent cells
  • Cells in solution
  • Co-cultures of cells on feeder layers
  • Range of labelling techniques

Human iPS cells

  • Screening on a highly relevant genetic background
  • In-house expertise in producing human iPS cell-derived neurons

High-content microscopy

  • Investigation of large sets of compounds or genes in many functional dimensions, linking them to biological parameters of interest (e.g. neurite outgrowth, synapse formation, or more complex interdependent processes)
  • ThermoScientific ArrayScan HCS Reader: neuron survival and dendrite morphology
  • PerkinElmer Opera LX confocal high-content microscopy system: synapse number and morphology
  • Accurate single cell measurements as well as population features
  • more than 40 morphological parameters relevant for neurological and psychiatric disease

High-content analysis of synaptic transmission

  • Patch clamp physiology on isolated single rodent or human neurons (autaptic cultures)
  • More than 30 physiological parameters that reflect key synaptic properties (e.g. evoked response amplitude and charge, mini frequency and amplitude, readily releasable pool size, Ca2+-dependent recruitment)

CYTOSPECTOR

  • Home
  • Expertise
  • High-content assays
  • Standardized phenotypic assay
  • Alzheimer´s disease assay
  • Assay development
  • About us
  • Contact

CONTACT

Cytospector
PO Box 71033
1008 BA Amsterdam
The Netherlands

info@cytospector.com

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