Assay Development
On demand, Cytospector develops neuron-based high-content assays for compound screens and screens for therapeutic targets in the field of neurological and psychiatric disease. Combination of advanced neuronal cell models, standardized analysis of a large number of relevant neuronal and synaptic phenotypes, and our long-standing expertise with high-content screening ensures optimal assay development.
Contact us with any questions or requests for assay development.
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Cytospector’s foundations for assay development
Primary rodent neurons
- High-throughput, fully automated cell culture
- Adherent cells
- Cells in solution
- Co-cultures of cells on feeder layers
- Range of labelling techniques
Human iPS cells
- Screening on a highly relevant genetic background
- In-house expertise in producing human iPS cell-derived neurons
High-content microscopy
- Investigation of large sets of compounds or genes in many functional dimensions, linking them to biological parameters of interest (e.g. neurite outgrowth, synapse formation, or more complex interdependent processes)
- ThermoScientific ArrayScan HCS Reader: neuron survival and dendrite morphology
- PerkinElmer Opera LX confocal high-content microscopy system: synapse number and morphology
- Accurate single cell measurements as well as population features
- more than 40 morphological parameters relevant for neurological and psychiatric disease
High-content analysis of synaptic transmission
- Patch clamp physiology on isolated single rodent or human neurons (autaptic cultures)
- More than 30 physiological parameters that reflect key synaptic properties (e.g. evoked response amplitude and charge, mini frequency and amplitude, readily releasable pool size, Ca2+-dependent recruitment)